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KMID : 0359320030430010151
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Korean Journal of Veterinary Research
2003 Volume.43 No. 1 p.151 ~ p.156
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Effect of high concentrations of carboxymethyl chitosan on preventing formation of peritoneal adhesion in rats
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Abstract
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The objective of this study was to determine the effective concentration of carboxymethyl chitosan(CMC) solution on preventing intraperitoneal adhesions. In this study, 60 rats were divided into four groups : an untreated control group and three experimental groups that were treated with 3 ml of 3, 5, and 7% CMC solution. Adhesions were induced by suturing both the ideal serosa and peritoneum scarped until petechial bleeding occurred. CMC solutions were instilled intraperitoneally immediately before closure until petechial bleeding occurred. CMC solutions were instilled intraperitoneally immediately before closure of the peritoneomuscular layer. The tensile strength of formed adhesions was measured by using a computerized tensiometer. Histopathological changes of adhesions were evaluated by hematoxylin-eosin for light microscopic examination and Massons´s trichrom staining for collagen fibers. All of CMC solutions reduced significantly the tensile strength of the adhesions. CMC inhibited the increase of inflammatory cell infiltration and damage of adhesion spot, and accelerated the collagen synthesis and neovascularization. No significant differences were observed among all of CMC concentration at inhibiting adhesion formation. Therefore, we could conclude that 3% CMC, the lowest concentration adopted in this study, was appropriate to prevent the formation of peritioneal adhesion.
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